My team’s goal is understanding the mechanisms and protein-protein interactions of the BCL-2 family of proteins in cell death and cell survival signaling pathways. We use MST for probing these molecular interactions and getting binding affinities because of the sensitivity of the method. This has benefited us particularly because our proteins are dynamic and prone to aggregation at the high concentrations required for other methods. With MST we can get affinities from proteins-protein and protein-small molecules interactions more reliably. We also use Tycho for routine check of the quality of our samples and MST assay development. Tycho is the perfect addition to the workflow because of its speed and label-free measurements.
Professor Evripidis Gavathiotis, Ph.D.
Departments of Biochemistry, Medicine and Experimental Therapeutics
Albert Einstein Cancer Center