We use NanoTemper Technologies’ Microscale Thermophoresis to characterize the affinity of our compounds to their targets. Using this technology we obtained very convincing data that were in agreement with results obtained using other technologies like Surface Plasmon Resonance (SPR). We appreciated the relatively short time for the time to establish the assay compared to alternative methods to measure protein affinities as well as the comparatively low amounts of protein consumption. We conclude that this new technology gives rise to reliable quantitative affinity data.
Dr. Alexandra Matzke