We used Microscale Thermophoresis (MST) to measure the interaction between large molecular chaperones with molecular weight up to 800 kDa. It is distinguished in low sample consumption, fast measurement and immobilization-free setup. We found that the biophysical data obtained from MST are in good agreement with those obtained from SPR or ITC. MST technology will facilitate our research on various interactions.
Prof. Cuimin Liu
Institute of Genetics and Developmental Biology
Chinese Academy of Sciences