MicroScale Thermophoresis (MST) is an extremely valuable tool to assess and quantify interactions. In one study using MST, we determined the binding affinities of a wild-type protein and 11 mutants in a half day; this would have been impossible with other techniques. We are using the Monolith NT.115 in our daily research work and we are very satisfied by the data obtained with this novel biophysical method. In addition, we appreciate the support and the competence from the NanoTemper Technologies Company.
Dr. D. J. Hart
European Molecular Biology Laboratory (EMBL)