The new 2nd Generation labeling kits from NanoTemper Technologies have improved the quantification of our measurements of intermolecular interactions and optimizing signal-to-noise ratios in our binding assays. In general, the kits provide a quick and reliable option for labeling our proteins without any loss. The His-Tag labeling kit Red-tris-NTA 2nd Generation is one of the preferred consumables used by our visitors at the EMBL Biophysics Core Facility. We have seen optimal and reproducible binding results using this labeling strategy. In summary, we highly recommend the 2nd Generation dyes, which have helped us to execute several MST measurements in a short, and reliable matter using a very small amount of our purified proteins.
Dr. Maria Garcia
European Molecular Biology Laboratory