MicroScale Thermophoresis (MST) by NanoTemper Technologies is very useful tool to quantify biomolecular interactions. The advantage of this platform is that the assay is simple to set up, it requires minimal reagents and biomolecular interactions can be measured under native conditions with minimal alterations to reactants. At Center for Chemical biology and Therapeutics, inStem, National center for biological sciences (NCBS), we have been using MST routinely to measure the protein-small molecule interactions across multiple protein domains successfully as an alternative to rather cumbersome approaches such as ITC, SPR and TSA. It is quick, precise and highly informative in reading the molecular interactions.
Dr. Muralidhara Padigaru
Institute for Stem Cell Biology and Regenerative Medicine