Jagiellonian University – Dr. Jacek Plewka – Prometheus

We are using nanoDSF to determine refolding conditions on proteins purified from inclusion bodies. Before getting the Prometheus, this was more of a trial and error process. Thanks to Prometheus, we can increase the number of test conditions from a few to almost 50 using the same amount of test protein. Also, Prometheus saves us a considerable amount of time as we focus the later analytical SEC testing only on the protein samples from successful conditions.


 

Jacek Plewka

Dr. Jacek Plewka
Post-doc
Jagiellonian University, Department of Chemistry
Kraków, Poland