Our recent research has focussed on the interactions of proteins involved in the Hedgehog signalling pathway. We have used MST to develop a binding assay with fluorescently labelled peptide and unlabelled protein. The speed of the assay and low sample consumption has allowed us to quickly identify the critical residues in the binding site by rapidly screening a series of mutated proteins. Furthermore, the assay can easily be adapted to screen peptides and small molecules for competitive binding.
Dr. Luca Jovine