For the Biochemistry Core Facility at the Max-Planck-Institute for Biochemistry it is of great importance to provide our users with instruments which are easy to use while offering a high information content. Therefore, the NanoTemper Monolith NT.115 with its low sample consumption and maintenance-free design is a perfect addition to our instrument park. The straight forward handling of the instrument allows even first time users to access binding affinities usually within a day. Method development is often quick, certainly compared to other techniques, but the method puts a high demand on sample homogeneity and in particular on monodispersity. As interactions are characterized free in solution, MST has been successful where SPR failed due to the limitations of a surface based approach.
Dr. Stephan Uebel
Max-Planck-Institute of Biochemistry