Memorial Sloan-Kettering Cancer Center – Dr. Gerard Minuesa

My main research at Sloan-Kettering Institute was based on biomolecular interactions between RNA-binding proteins and RNA and the discovery of small-molecules targeting that specific interaction. After optimizing and running FP-based high-throughput screen for small-molecules, the best and most useful technique to validate our hits was in fact MST with Monolith NT.115. Initially, we picked MST over other techniques for its versatility and high sensitivity, but later on we also benefited from the low sample size (protein and RNA), the easy set up and the very user-friendly analysis software. All of which meant we could achieve our research goals at a much faster pace. Remarkably, in few weeks we could perform optimal RNA-protein, RNA-small molecule and protein-small molecule interactions with very little assay development, labeling both nucleic acids and proteins with. Some of our MST data can be seen in Minuesa G. et al. Nat Commun. 2019, 19; 10(1):2691


 

Gerard Minuesa

Dr. Gerard Minuesa
Former Research Associate
Memorial Sloan-Kettering Cancer Center