Our lab focuses on the importance for telomere and telomerase to maintain the integrity of genomes and the stability of chromosomes. Large amount of proteins and protein complexes are involved in epigenetics, which play essential rotes in controlling many cellular events. We are employing MicroScale Thermophoresis (MST) in determining the interactions of proteins/protein complexes to ssDNA/RNA. It’s easier to measure the protein-protein or protein-nucleic acid interaction, especially for those sensitive samples, that has been difficult to detect and quantify with ITC or SPR. We benefited from the low sarnple consumption, the fast measurements, the broad application range and the possibility to choose the buffer freely, resulting in highly reproducible data. We strongly recommend MST technology for its low material consumption, short measurement times and very high sensitivity.
Prof. Dr. Lei Ming
Director, National Center for Protein Science Shanghai (NCPSS)
Deputy Director, Shanghai Institute of Biochemistry and Cell Biology (SIBCB)