Cryo EM is a powerful method for gaining structural insights about molecules and their functional complexes in a solution. It is particularly important for understanding mechanisms of action of previously non-crystalizable protein targets. Such targets often prove to be difficult to handle and there is a strong need to verify the quality of sample entering CryoEM analysis. Tycho NT.6 is a great addition to our Cryo EM toolbox. It provides a fast and effortless way to analyze protein quality. It can be used at various stages preceding Cryo EM analysis to verify functionality of proteins – including protein purification steps, storage analysis or optimal buffer selection.
Dr. Sebastian Glatt
Head of the National Cryo-EM Facility, NSRC Solaris
Max Planck Research Group Leader, Malopolska Centre of Biotechnology
Deputy Director for Science, Malopolska Centre of Biotechnology
Jagiellonian University Krakow, Poland