For such weak interactions [millimolar to micromolar], it is difficult to set up reliable biochemical assays or binding assays using ITC or SPR techniques. Fortunately, MST technology turns out to be an excellent way to determine such weak interactions between small fragments and protein in our system of interest. We efficiently and reliably performed our experimental binding measurement using minimum amount of protein and fragments. We strongly recommend MST technology for its low material consumption, short measurement time, and very high sensitivity.
Dr. Niu Huang