MST allows us to easily explore the binding sites of plant enzymes and supplements classical kinetics measurements used in enzymology studies such as Kcat, Km or Ki, with the Kd. It also provides crucial information from site-directed mutagenesis experiments, when the key catalytic residues are targeted and the classical assays to determine kinetic parameters fail due to negligible or no activity of the mutants. With MST we can easily find out whether the mutation results only in loss of catalytic activity or binding affinity or potentially both.
Dr. David Kopecny
Department of Protein Biochemistry and Proteomics