Our Monolith NT.115 instrument is a fundamental piece in a set of equipment we use regularly to characterize protein-protein, protein-small molecule and protein-lipid interactions. In order to obtain reliable estimates of binding strength and stoichiometry, we regularly utilize quartz crystal microbalance (QCM), surface plasmon resonance (SPR), and isothermal titration calorimetry (ITC) techniques. MicroScale Thermophoresis (MST) is indispensable for us to measure precious or labor-intensive samples not suitable for ITC because of the required quantity and purity. We found MST suitable for fragment-based design and quality control of biologics development as well. We plan to expand our instrument range with Monolith NT.LabelFree and Seismos in the future. We are very satisfied with the support of NanoTemper and the competence of the company’s application specialists.
Dr. Karoly Liliom
Institute of Enzymology
Research Centre for Natural Sciences
Hungarian Academy of Sciences