In our group we apply protein engineering methods to optimize, for example, the activity and/or thermal stability of various biocatalysts. After rational or random mutagenesis, it is critical to verify the structural integrity and stability of the resulting enzyme variants. For this purpose, nanoDSF is really helpful as it enables an accurate and reliable measurement of melting temperatures of a large numbers of variants. We have also employed nanoDSF to test the influence of substrates, buffers, additives and cosolvents on protein stability of these biocatalysts. Importantly, the simple capillary filling procedure and intuitive software make the device easy to use, even for beginners.
Dr. Dominique Böttcher
Department of Biotechnology & Enzyme Catalysis
University of Greifswald