The Researcher’s Guide to Measuring Binding Affinity and Why it Matters

Whether you’re just starting to learn about measuring binding affinity or you’re looking for a refresher, this guide covers why measuring binding affinity is key to making the right decisions about what to do next in your research. You’ll also discover available technologies and helpful tips to evaluate them before purchasing.

The Scientist’s Guide to Characterizing Protein Stability

Measuring protein stability is necessary for numerous applications from understanding the molecular basis for certain diseases to developing more effective drugs. This guide has some helpful tips to answer why characterizing a protein’s stability is important, what technologies are available and how to thoroughly evaluate them before purchasing.


What’s inside

How measuring binding affinity can inform your research
From life science research to drug discovery, understanding the strength of a bond between a single molecule to its ligand, known as affinity, has become increasingly important. Learn how it can inform your research decisions.

When to measure binding affinity
In academics and industry, measuring the affinity is usually performed as one of the last experiments or when pre-clinical work is over. In some cases, it may be beneficial to measure this upstream. Determine when it is right for you.

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What’s inside

Why measuring protein stability is necessary
Understanding proteins stability is key to gaining insights into the function, regulation, and regulatory roles of a molecule. From describing cellular pathways in certain diseases to applications such as screening and developing candidates for new drugs, learn how characterizing a protein’s stability informs your research decisions.

How to characterize protein stability
To measure a protein’s stability, you need to look at how it unfolds. Learn about the two common methods used to cause protein unfolding – thermal and chemical – and what parameters you should be measuring.

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Helpful tips you can use right away

Which techniques and technologies are best for you
There are several dedicated techniques and technologies that enable the characterization of protein stability and the measurement of binding affinity. They all have different strengths and weaknesses. Learn what they are and discover the right one for your research.

What to think about when evaluating technology
There is more than just the price tag to consider when finding an instrument. With a helpful step-by-step buyer’s checklist, learn practical tips on evaluating your research needs, exploring vendors, and getting your organization on board.

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