To investigate biomolecular interactions, my group uses a wide range of techniques. MicroScale Thermophoresis (MST) is mainly used as a fast approach for the initial characterization of interactions. Advantages include low material consumption, short measurement times and a wide range of accessible interactions. Kd values obtained with MST are generally in good agreement with those obtained using ITC or Ki values determined with the help of enzymatic assays.
Prof. Dr. Dietmar Manstein
Hannover Medical School