When binding affinity matters
Swiftly measure binding interactions and get accurate Kd results using very little sample with the Monolith system
Get results quickly
Capture measurements in minutes not hours or even days. Collect more information and make better decisions on what to do next, sooner.
Measure any sample type
Easily measure binding interactions between molecules of different sizes, molecular weights or pM to mM binding affinities.
Consume very little sample
Generating sample is already hard enough and using it all up for analysis is heartbreaking. Get Kd values with just a tiny amount of sample.
Do minimal sample prep
Saving a bit of time here and there adds up so the less time prepping samples, the better. Analyze samples in any buffer or bioliquid and in close-to-native conditions. Oh, and purification or immobilization isn’t needed either.
Optimize assays easily
Feedback is immediate and doesn’t take days or weeks to get up and running—this makes optimizing assays so much easier.
Evaluate the most difficult targets
Running into a roadblock? Get results even when other methods don’t work.
When protein stability matters.
Prometheus precisely characterizes thermal unfolding, chemical denaturation and aggregation in a single run with the most flexibility a system has to offer
Start with the winner—Get reproducible, quantitative results early on from discovery to validation and production. Use Prometheus to generate precise unfolding temperatures (Tm and Tonset), critical denaturant concentrations (Cm), free folding energy (ΔG and ΔΔG), and aggregation results (Tagg) from start to finish. Be confident about moving forward with the right choice every step along the way.
Measure under native conditions
This is the ideal situation – no dyes are needed, neither is sample dilution or alternate buffer conditions. Running experiments with viscous samples is also not an issue.
Consume less sample
Samples are precious so using less to do more is always the goal. Requiring as little as 10 µL in volume and 5 µg/mL in concentration is always a plus.
Prep samples… not
Wouldn’t it be great to just quickly load samples and get a result in minutes? No sample prep needed, just load as-is.
Test more conditions
The flexibility to design experiments by testing multiple parameters in one run makes it easier to get to the end goal sooner.
Get precise results
A high density of data points means better data quality and seeing details missed by other technologies.
Choose any throughput
Decide to run 1, 48 or any number of samples in between and do only what’s necessary. Or, choose to automate and do more.
Do more simultaneously
Why set up multiple experiments when it can be taken care of with one? Get Tm, Tonset and Tagg results all in one run and end up spending time on other things.
Use in regulated environments
Prometheus supports 21 CFR Part 11 compliance, so feel free to use it wherever whenever.