What binding affinity and binding kinetics tell you about protein-ligand interactions

What measurements are most important for studying how proteins interact? The answer depends on your research needs. There is debate about which is more important: measuring how fast and specific the interaction is between two biomolecules (binding kinetics), or the strength between them (binding affinity). Real-time measurements like surface plasmon resonance (SPR) provide binding affinity and kinetics, but does that mean researchers always need it? Delving into the differences in protein measurement types and current instrument technologies can help you determine which one will best serve your research purposes.