Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

Maureen McKeague, Ranganathan Velu, Annalisa De Girolamo, Stefania Valenzano, Michelangelo Pascale, McKenzie Smith and Maria C. DeRosa

Toxins
2016 vol: 8 issue: 11 pp: 336 doi: 10.3390/toxins8110336

Abstract

Ochratoxin A (OTA) is a mycotoxin produced as a secondary metabolite by several species of Aspergillus and Penicillium and frequently found as a natural contaminant in a wide range of food commodities. Novel and robust biorecognition agents for detecting this molecule are required. Aptamers are artificial nucleic acid ligands able to bind with high affinity and specificity to a given target molecule. In the last few years, three separate research groups have selected aptamers for ochratoxin A. While each of these three families of aptamers have been incorporated into various methods for detecting OTA, it is unclear if each aptamer candidate is better suited for a particular application. Here, we perform the first head-to-head comparison of solution-based binding parameters for these groups of aptamers. Based on our results, we provide recommendations for the appropriate choice of aptamer for incorporation into solution-based biorecognition assays and applications.

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Topics: Aptamer, Ochratoxin A, Mycotoxins, Biorecognition, Fluorescent assay, Biosensing, Aptasensor, Monolith – MicroScale Thermophoresis, MST, Proteins, Publications