Chromatin, the packaging form of the genome possesses two fundamental functions. On the one hand it compacts the DNA to fit into the nucleus and on the other hand it allows access to the underlying sequences for essential DNA dependent processes. Nucleosomes represent the basic structural component of chromatin. Here we characterize the specific interaction of the Decondensation factor 31 (Df31) with mono-nucleosomes. Df31 was recently shown to form a complex with snoRNAs and chromatin, to generate accessible higher order structures of chromatin.