How to Study Protein-protein Interactions

Marjetka Podobnik, Nada Kra{evec, Apolonija Bedina Zavec, Omar Naneh,
Ajda Flaker, Simon Caserman, Vesna Hodnik1, and Gregor Anderluh1

Slovenian Chemical Society
2016 vol: 63 issue: 3 pp: 424–439 doi: 10.17344/acsi.2016.2419


Physical and functional interactions between molecules in living systems are central to all biological processes. Identification
of protein complexes therefore is becoming increasingly important to gain a molecular understanding of cells
and organisms. Several powerful methodologies and techniques have been developed to study molecular interactions
and thus help elucidate their nature and role in biology as well as potential ways how to interfere with them. All different
techniques used in these studies have their strengths and weaknesses and since they are mostly employed in in vitro
conditions, a single approach can hardly accurately reproduce interactions that happen under physiological conditions.
However, complementary usage of as many as possible available techniques can lead to relatively realistic picture of the
biological process. Here we describe several proteomic, biophysical and structural tools that help us understand the nature
and mechanism of these interactions.

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Topics: Cell signalling, Molecular interactions, Yeast two-hybrid, Surface plasmon resonance, Quartz crystal microbalance, Structural biology, Monolith – MicroScale Thermophoresis, MST, Proteins, Publications