On-Demand Webinar: Identifying novel biocatalyst candidates via high-throughput stability monitoring
The stability of enzymatic biocatalysts is of primary importance in the biotechnology, food, chemical, and pharmaceutical industries, but screening for biocatalyst stability has been a long-standing challenge. Native enzymes may not possess the optimal activity required for a specific protein-engineering need, which is why biocatalysts are often modified to withstand different conditions, such as elevated temperatures or the presence of organic co-solvents. In this webinar, learn about Prometheus, a platform that runs nanoDSF technology and how Prof. Wolf-Dieter Fessner from Technische Universität Darmstadt is using this system to more efficiently screen biocatalyst candidates by characterizing their stability. He will share how nanoDSF can reveal key information on the stability of these enzymes and assist in more effectively identifying and selecting these target molecules.
Topics to be covered:
- Identifying and isolating biocatalysts with optimal properties based on their stability
- Benefits to understanding target molecule stability in selection, optimiziation, and characterization steps
- Analysis of protein stability using nanoDSF
About the Speaker
Prof. Wolf-Dieter Fessner
Technische Universität Darmstadt
“Woody” Fessner obtained his Ph.D. in 1986 and worked as a postdoctoral fellow with George Whitesides (Harvard University) and George Olah (USC, L.A.). Before assuming his current position as full professor of organic chemistry at the Technische Universität Darmstadt in 1998, he was a professor at RWTH Aachen. Starting with the 1992 landmark development of in vitro multi-step “Artificial Metabolisms”, his research interests are in the area of biocatalysis for applications in organic synthesis, with particular emphasis on the discovery and development of novel enzymes for stereoselective carbon-carbon bond forming reactions and the synthesis of complex oligosaccharides. His group is also interested in the application of directed evolution technologies for the development of biocatalytic modules with functions specifically tailored for cascade applications in Systems Biocatalysis. Currently, he is coordinating the Horizon2020 project CarbaZymes and the ERA CoBioTech project Tralaminol.