Interaction of maltose binding protein (MBP) with maltose in a truly label-free assay

Protein-sugar interactions are essential for nutrient transport and metabolism in all types of cells. Here, we investigate the interaction of maltose binding protein (MBP) and its substrate, maltose, drawing upon label-free MicroScale Thermophoresis (MST) as the ideal technique. We also show that sample viscosity did not affect the binding assay, and discuss how MST is not limited by sample viscosity.