One-step, purification-free and site-specific labeling of polyhistidine-tagged proteins for MST
NanoTemper Technologies uses the popular polyhistidine-tag as target for an unparalleled one step, purification-free labeling of proteins for MST experiments. The labeling strategy employs a high affinity multivalent nitrilotriacetic acid (NTA) derivative conjugated with the MST-optimized NT-647 dye (RED-tris-NTA). As the hexahistidine tag (His6) provides binding sites for three NTA moieties, RED-tris-NTA is perfectly suited for non-covalent, stable, highly selective labeling with a 1:1 stoichiometric ratio. The labeling can be performed with minute amounts of either purified biomolecules or directly in the cell lysate.