Surface plasmon resonance (SPR) is an optical methodology used to detect and quantify molecular interactions. Although SPR is widely used and considered by many researchers to be the gold standard for quantification of protein interactions, assay optimization can be technically challenging, time-consuming and costly. The Tycho NT.6 system quickly analyzes different conditions typically tested when optimizing an SPR assay. It utilizes very small amounts of sample and provides dramatic time and cost savings compared to standard SPR assay development procedures. Tycho NT.6 is simple to use and enables researchers to make better educated decisions in developing and optimizing their binding interaction assays.