Quantifying CBM Carbohydrate Interactions Using Microscale Thermophoresis
Wu H, Montanier C, Dumon C
Methods in Molecular Biology
2017 vol: 1588
MicroScale Thermophoresis (MST) is an emerging technology for studying a broad range of biomolecular interactions with high sensitivity. The affinity constant can be obtained for a wide range of molecules within minutes based on reactions in microliters. Here, we describe the application of MST in quantifying two CBM-carbohydrate interactions, a CBM3a toward cellulose nanocrystals and a CBM4 against xylohexaose.
Topics: Ligand induced fluorescence change, Measure binding affinity, Monolith – Microscale Thermophoresis, Proteins, Publications, Saccharides