Rational design of a bisphenol A aptamer selective surface-enhanced Raman scattering nanoprobe

Haley L. Marks, Michael V. Pishko, George W. Jackson, and Gerard L. Coté

Analytical Chemistry
2014 vol: 86(23) pp: 11614–11619 doi: 10.1021/ac502541v

Abstract

Surface-enhanced Raman scattering (SERS) optical nanoprobes offer a number of advantages for ultrasensitive analyte detection. These functionalized colloidal nanoparticles are a multifunctional assay component. providing a platform for conjugation to spectral tags, stabilizing polymers, and biorecognition elements such as aptamers or antibodies. We demonstrate the design and characterization of a SERS-active nanoprobe and investigate the nanoparticles’ biorecognition capabilities for use in a competitive binding assay. Specifically, the nanoprobe is designed for the quantification of bisphenol A (BPA) levels in the blood after human exposure to the toxin in food and beverage plastic packaging. The nanoprobes demonstrated specific affinity to a BPA aptamer with a dissociation constant Kd of 54 nM, and provided a dose-dependent SERS spectra with a limit of detection of 3 nM. Our conjugation approach shows the versatility of colloidal nanoparticles in assay development, acting as detectable spectral tagging elements and biologically active ligands concurrently.

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Topics: Aptamers, Nucleotide/chemical synthesis, Aptamers, Nucleotide/chemistry, Benzhydryl Compounds/blood, Drug Design, Humans, Molecular Structure, Nanoparticles/chemistry, Phenols/blood, Spectrum Analysis, Raman, Surface Properties, Monolith–MicroScale Thermophoresis, MST, Proteins, Publications