Binding Affinity

The Binding Affinity templates in DI.Control software can be used to quantify any molecular interaction between a fluorescently labeled Target molecule and a corresponding Ligand molecule. Before a binding affinity experiment, a Binding Check experiment is highly recommended to establish the assay conditions and to determine whether an interaction can be observed. Following this, a Binding Affinity experiment is performed to monitor the target’s TRIC signal over a dilution series of the ligand in order to derive a binding affinity (K_d or EC₅₀).