The term on-time is used to describe two closely related, but different aspects of a TRIC measurement. Laser on-time on signifies the time period during which the infrared (IR) laser is active and generating a local temperature change in the sample (in DI.Control software, the on-time to be set to either 3 or 5 sec). The TRIC on- time also signifies the specific time point at which binding analysis is performed for affinity data. As an example, in a typical measurement the IR-laser is active for 5 sec laser on-time but data analysis can be performed afterwards with the F1 cursor set to 3 sec TRIC on-time for calculation of Fnorm data. In DI.Screening Analysis software the Response Evaluation Mode determines the on-time selected for analysis.