标记度计算器
如果您使用的是 NanoTemper Monolith蛋白标记试剂盒，此计算器可以帮助您根据分光光度计测量的吸光度值计算荧光标记蛋白的标记度 （DOL）。
您的结果意味着什么？
What’s DOL?
The DOL describes how many dye molecules are bound to your protein, e.g. a DOL of 1 refers to a dye:protein ratio of 1:1. If the DOL is between 0.5 and 1, you should record this number and continue on with your MST measurement.
What should the DOL be?
Ideally, it should be between 0.5 and 1. We recommend you record your DOL every time you label your protein and aim to obtain a similar DOL to achieve consistent MST results.
对于大多数蛋白质，应避免DOL大于 1因为它会对蛋白质功能产生不利影响。这意味着某些蛋白质至少标记上了 2 个染料分子（过度标记），您需要降低染料与蛋白质的比例。
应避免DOL低于 0.5，因为它会导致信噪比降低。这意味着您的蛋白质很可能没有被标记，您需要增加染料与蛋白质的比例。
Why am I being told my protein’s concentration (I came here to calculate DOL)?
Well to calculate DOL, we needed to calculate the protein concentration too. So instead of just keeping your protein’s concentration to ourselves, we thought you might like to know it as well!
