静态光散射技术（SLS）

初始散射值 is a quick quality indicator about your sample. When developing well-folded, uniform proteins for therapeutics, it is important to avoid aggregation or large particle contaminations. If the scattering value is very high, it is an indicator that large particles are contaminating your protein sample. If it is very low, your sample’s concentration may not be high enough for subsequent DLS experiments.

分子量 MW, derived from SLS is the average mass of particles in solution. 

Second virial coefficient, B₂₂, is a parameter that uses scattering to determine the self-association propensity of a sample. If proteins are likely to self-associate – indicating a tendency towards high viscosity or aggregation as the sample becomes more concentrated. High concentrations are required for clinical administration, so researchers must avoid negative indicators of self-association, either by optimizing the construct or its buffer environment.