Start with the winner
Take assumptions out of the equation—Get reproducible, quantitative results early on from discovery to validation and production. Use Prometheus to generate precise unfolding temperatures (Tm and Tonset), critical denaturant concentrations (Cm), free folding energy (ΔG and ΔΔG), and aggregation results (Tagg) from start to finish. Be confident about moving forward with the right choice every step along the way.
Measure under native conditions
This is the ideal situation – no dyes are needed, neither is sample dilution or alternate buffer conditions. Running experiments with viscous samples is also not an issue.
Consume less sample
Samples are precious so using less to do more is always the goal. Requiring as little as 10 µL in volume and 5 µg/mL in concentration is always a plus.
Prep samples… not
Wouldn’t it be great to just quickly load samples and get a result in minutes? No sample prep needed, just load as-is.
Test more conditions
The flexibility to design experiments by testing multiple parameters in one run makes it easier to get to the end goal sooner.
Get precise results
A high density of data points means better data quality and seeing details missed by other technologies.
Choose any throughput
Decide to run 1, 48 or any number of samples in between and do only what’s necessary. Or, choose to automate and do more.
Do more simultaneously
Why set up multiple experiments when it can be taken care of with one? Get Tm, Tonset and Tagg results all in one run and end up spending time on other things.
Use in regulated environments
Prometheus supports 21 CFR Part 11 compliance, so feel free to use it wherever whenever.
Flexibility with Prometheus
Little to no experience is needed when it comes to measuring protein stability and protein aggregation on the Prometheus. Each system has a friendly lab footprint and is maintenance-free. The only tough decision is which throughput to choose.
Test more conditions with flexible throughput
Scale up for higher throughput
Prometheus NT.Plex plus NT.Robotic Autosampler
Get fully automated, screening capabilities
The nanoDSF instrument has become one of the primary techniques to screen for the thermal stability of globular proteins at Novo Nordisk A/S. The instrument and software are intuitive and easy to handle which makes this suitable as a walk-up instrument. The method is very versatile due to fluorescence and aggregation optics, low sample consumption, concentration range and insensitivity towards many buffers and excipients. For globular proteins with tryptophan residues we yield high quality thermal unfolding data which compare with other conventional methods.
Dr. Nikolai Lorenzen, Novo Nordisk A/S, Denmark
Higher quality results, better decisions
Prometheus does it best when it comes to characterizing protein unfolding. In both thermal and chemical denaturation studies, even the most subtle unfolding events are easily seen that other traditional systems simply can’t detect. Best of all, results are not compromised by aggregates in solution. Get higher quality results and gain the ability to make better decisions.
Prometheus monitors the intrinsic fluorescence signal of proteins as a measure of its folding state. A ratiometric measurement of the fluorescent signal is plotted against increasing temperature or concentration of a chemical denaturant to determine the Tm of a protein.
Predict stability with confidence
Being confident in what to do next is key to doing great research. The formulation development group at Boehringer Ingelheim realized that having accurate, precise and high quality data for both thermal unfolding and aggregation is key to better predicting stability, developability and longer-term storage of their antibody candidates.
Prometheus provides comprehensive results for any type of protein—small or large molecules, biologics, enzymes, antibodies, ADCs and membrane proteins—and is especially good at screening buffer influences or testing formulation and storage conditions. Make better decisions based on more complete stability results.
Effects of pH on the thermal unfolding and aggregation propensity on a mAb target protein
Small amounts, low or high concentration, viscous? No problem.
Prometheus characterizes thermal and chemical unfolding under native conditions using nanoDSF technology. Absolutely label-free, nanoDSF precisely measures the intrinsic fluorescence of a protein while it’s being subjected to either chemical or thermal denaturation.
nanoDSF uses high quality capillaries for a number of reasons—only microliters of sample are required, a wide range of concentrations are quickly measured in-solution, multiple conditions are assessed in one run, and hard-to-measure viscous samples are examined with ease.
Just dip a capillary into a sample, place it in Prometheus, and hit start to get Tm, Tonset and Tagg results in minutes. And, if Cm, ΔG and ΔΔG are needed, those can be done too with the same system.
Software that’s just right
It’s so difficult to find a system that’s easy to use these days while also providing flexible measurement options. Prometheus uses PR.ThermControl, PR.ChemControl or PR.TimeControl software, all of which guide researchers step-by-step through each experiment making assay setup a so
And, for those times when you’re looking to compare runs and merge replicates to see if your results are statistically relevant, use PR.Stability Analysis to help you draw more precise conclusions.
Precisely determine at which temperature 50% of your protein unfolds (Tm) and when aggregation begins (Tagg) with PR.ThermControl. Since assay setup is 3 steps only and there’s few experimental parameters to set, you’ll be off and generating data quicker than you imagine. Results are automatically generated and color coded so you can easily visualize the results. Learn more
Use PR.ChemControl when you want to evaluate the chemical stability of a protein. It only takes 40 seconds to get data on the energetics of folding (ΔG) and the denaturant concentration at which 50% of the protein is unfolded (Cm). It’s great for getting quick answers on chemical treatments or conditions that influence protein unfolding and stability.
Are you searching for more details on the rates of unfolding and refolding? Maybe you’re finding a need to run more advanced thermal profiles such as isothermal measurements or a thermal cycling. You’ll want to have PR.TimeControl. Your ability to customize thermal treatment assay conditions is one of the best reasons to use it. Feel free to choose between isothermal measurements, incremental temperature cycling or temperature stepping for those advanced protein stability measurements you’ve always wanted to do.
If making more precise conclusions about the stability of your proteins is your ultimate goal then there’s no better way to do that than with PR.Stability Analysis software. Use it to quickly visualize and identify any key trends by sorting, filtering or plotting data as you need. Choose to merge replicates or compare runs. Then conveniently export your results in ready-made formats for bookkeeping, presentation or publication. Learn more