Start with the winner
Take assumptions out of the equation—Get reproducible, quantitative results early on from discovery to validation and production. Use Prometheus to generate precise unfolding temperatures (Tm and Tonset), critical denaturant concentrations (Cm), free folding energy (ΔG and ΔΔG), and aggregation results (Tagg) from start to finish. Be confident about moving forward with the right choice every step along the way.
Measure under native conditions
This is the ideal situation – no dyes are needed, neither is sample dilution or alternate buffer conditions. Running experiments with viscous samples is also not an issue.
Consume less sample
Samples are precious so using less to do more is always the goal. Requiring as little as 10 µL in volume and 5 µg/mL in concentration is always a plus.
Prep samples… not
Wouldn’t it be great to just quickly load samples and get a result in minutes? No sample prep needed, just load as-is.
Test more conditions
The flexibility to design experiments by testing multiple parameters in one run makes it easier to get to the end goal sooner.
Get precise results
A high density of data points means better data quality and seeing details missed by other technologies.
Choose any throughput
Decide to run 1, 48 or any number of samples in between and do only what’s necessary. Or, choose to automate and do more.
Do more simultaneously
Why set up multiple experiments when it can be taken care of with one? Get Tm, Tonset and Tagg results all in one run and end up spending time on other things.
Use in regulated environments
Prometheus supports 21 CFR Part 11 compliance, so feel free to use it wherever whenever.
Flexibility with Prometheus
Little to no experience is needed when it comes to measuring protein stability and protein aggregation on the Prometheus. Each system has a friendly lab footprint and is maintenance-free. The only tough decision is which throughput to choose.
Test more conditions with flexible throughput
Scale up for higher throughput
Prometheus NT.Plex plus NT.Robotic Autosampler
Get fully automated, screening capabilities
The nanoDSF instrument has become one of the primary techniques to screen for the thermal stability of globular proteins at Novo Nordisk A/S. The instrument and software are intuitive and easy to handle which makes this suitable as a walk-up instrument. The method is very versatile due to fluorescence and aggregation optics, low sample consumption, concentration range and insensitivity towards many buffers and excipients. For globular proteins with tryptophan residues we yield high quality thermal unfolding data which compare with other conventional methods.”
Dr. Nikolai Lorenzen, Novo Nordisk A/S, Denmark
Higher quality results, better decisions
Prometheus does it best when it comes to characterizing protein unfolding. In both thermal and chemical denaturation studies, even the most subtle unfolding events are easily seen that other traditional systems simply can’t detect. Best of all, results are not compromised by aggregates in solution. Get higher quality results and gain the ability to make better decisions.
Prometheus monitors the intrinsic fluorescence signal of proteins as a measure of its folding state. A ratiometric measurement of the fluorescent signal is plotted against increasing temperature or concentration of a chemical denaturant to determine the Tm of a protein.
Predict stability with confidence
Being confident in what to do next is key to doing great research. The formulation development group at Boehringer Ingelheim realized that having accurate, precise and high quality data for both thermal unfolding and aggregation is key to better predicting stability, developability and longer-term storage of their antibody candidates.
Prometheus provides comprehensive results for any type of protein—small or large molecules, biologics, enzymes, antibodies, ADCs and membrane proteins—and is especially good at screening buffer influences or testing formulation and storage conditions. Make better decisions based on more complete stability results.
Effects of pH on the thermal unfolding and aggregation propensity on a mAb target protein
Small amounts, low or high concentration, viscous? No problem.
Prometheus characterizes thermal and chemical unfolding under native conditions using nanoDSF technology. Absolutely label-free, nanoDSF precisely measures the intrinsic fluorescence of a protein while it’s being subjected to either chemical or thermal denaturation.
nanoDSF uses high quality capillaries for a number of reasons—only microliters of sample are required, a wide range of concentrations are quickly measured in-solution, multiple conditions are assessed in one run, and hard-to-measure viscous samples are examined with ease.
Just dip a capillary into a sample, place it in Prometheus, and hit start to get Tm, Tonset and Tagg results in minutes. And, if Cm, ΔG and ΔΔG are needed, those can be done too with the same system.
Guidance throughout experiments
It’s so difficult to find a system that’s easy to use these days. Prometheus uses PR.ThermControl, PR.ChemControl and PR.TimeControl software, all of which guide researchers step-by-step through each experiment making assays so painless to set up. From the moment samples are loaded, measurement settings are simply optimized and executed. While the experiment is running, quickly name or color code samples, and when it’s done, results are instantly calculated. Choose to display the most important data at the end and easily export in one click to share with colleagues. Generating results can’t get any easier.