Move forward with the best candidates quickly

Reduce your overall development time by measuring binding affinity in minutes. Get accurate Kd results using very little sample.

Get results quickly
Capture measurements in minutes not hours or even days. Collect more information and make better decisions on what to do next, sooner.

Measure any sample type
Easily measure binding interactions between molecules of different sizes, molecular weights or pM to mM binding affinities.

Consume very little sample
Generating sample is already hard enough and using it all up for analysis is heartbreaking. Get Kd values with just a tiny amount of sample.

Do minimal sample prep
Saving a bit of time here and there adds up so the less time prepping samples, the better. Analyze samples in any buffer or bioliquid and in close-to-native conditions. And purification or immobilization isn’t needed either.

Optimize assays easily
Feedback is immediate and doesn’t take days or weeks to get up and running—this makes optimizing assays so much easier.

Evaluate the most difficult targets
Running into a roadblock? Get results even when other methods don’t work.

 


Measure with the Monolith

Anyone in the lab can measure binding affinity in minutes. Each system is simple to use and not only has a friendly lab footprint, but is completely maintenance-free.

 

Monolith NT.115

Most versatile
Crowd favorite for binding interactions

Monolith NT.115Pico

High sensitivity
Detect affinities in low pM range

Monolith NT.LabelFree

Dye-free
Analyze label-free using intrinsic fluorescence

Monolith NT.Automated

Workhorse
Fast screening with reliable results. Add the NT.Automated Screening Unit for fully automated sample and liquid handling

The Monolith NT.115 system is easy to use and only takes a short amount of time to set up experiments. The sample consumption is very low and its immobilization-free approach makes handling protein samples and setting up assays less complicated. The technical support and customer service from NanoTemper are excellent. Overall, we are very satisfied and strongly recommend the system to research groups looking for a fast quantitative binding assay.

Professor Antonio Macchiarulo
Department of Pharmaceutical Sciences, University of Perugia, Italy

 

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Be confident with precise results

Monolith is all about accurately determining binding affinity in minutes. The strength of the interactions between a fluorescently labeled or intrinsically fluorescent sample and a binding partner (or ligand) are measured while a temperature gradient is applied (gray box) over time (left figure). From this, binding affinity (Kd) is automatically calculated from a fitted curve that plots normalized fluorescence against concentration of ligand (right figure). Get answers for 1, 16 or even 96 samples at a time, and be confident that results are precise and complete.

 

Make the right decision with clear answers

More knowledge means more decision-making power. Determining binding affinity between large or small molecules, nucleic acids or even ions provides answers to important mechanisms of action or regulatory pathways.

For binding affinities, the lower the Kd, the higher the strength of the binding interaction between two molecules. By understanding this with the Monolith, Dong et. al. finally had a view into how cellular expression and regulation work in their CRISPR RNA model.

Monitoring MG2+ dependence of CRISPR RNA to a Cas9-like nuclease
Dong et. al., Nature, 532:522-536 2016

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Accuracy is what counts

Monolith uses MicroScale Thermophoresis (MST) technology to quantify binding events. One partner is typically labeled with a fluorescent dye and becomes an extremely sensitive reporter for all binding interactions. These events, weak or strong, cause a change in MST signal that is easily detected. The results are automatically translated into precise and quantitative Kd values.

Getting these accurate results quickly requires the use of high quality capillaries. Advantages are numerous—minute amounts of samples are analyzed in-solution, under native or close-to-native conditions, and in an immobilization-free environment without the need for purification.

 


Software that makes sense

Anyone in the lab can run binding interaction experiments—MO.Control software calculates all the volumes and dilutions needed to run an assay. With guided setup, real-time optimization tips, and built-in knowledge-based advice, users feel confident their experiments will run smoothly. MO.Control saves time by automatically calculating binding affinity values and instantly generating complete result summaries. Exporting data and reporting results is super easy with just one click.

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Get the best results with tailor-made consumables

Monolith capillaries are made with care—they’re made in a state-of-the-art facility and rigorously tested. Pair them with one of the Monolith Protein Labeling Kits and any of the Monolith systems to get the highest quality data and ultimately, the best outcome.

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