Quickly find and rank hits
Dianthus removes the complexity of binding interaction measurements for drug discovery screening. Get the fastest time to meaningful results with no immobilization required, because you demand it.
Find hits for any target type in any buffer or bioliquid
Consume the smallest amount of your target and library compounds
Measure the tightest interactions down to picomolar Kds
Tackle many projects, not just a few
Explore every target, even the most challenging ones
There’s no end to the projects you can do. Study targets of any size or mass — from ions to multimeric proteins — and type — proteins, nucleic acids, saccharides, and more.
Evaluate any sample type in any buffer
Dianthus isn’t picky. Feel free to prep your target in the buffer that ensures its stability and integrity. Even use lysates or bioliquids without diluting them.
Measure affinities with the highest sensitivity
Whether strong or weak, Dianthus detects a wide range of binding affinities — picomolar to millimolar — so you don’t have to use other methods.
Characterize in solution, no immobilization required
Analyzing interactions in close-to-native conditions is ideal. Dianthus characterizes in solution, so interfering with your target’s binding site or spending on expensive immobilizing chemistries isn’t an issue.
Consume small amounts of target and compounds
Every little bit counts. Saving on costly sample and library compounds means you can do more screening or use them in other projects.
Get meaningful results faster at any throughput
Dianthus swiftly analyzes 384 data points in 30 minutes and has flexible throughput. Choose to run one sample or as many as you want.
Use Dianthus 24/7
With Dianthus, there’s no fluidics. That means regular maintenance is not required ➡️ which means there’s no downtime ➡️ which means you can use it 24/7 whenever you want. Since Dianthus uses a 384-well microplate format, it’s compatible with many automation solutions and easily fits into your current workflow for even more hands-free time.
Fast time to results and nanomolar sensitivity
Fast time to results with highest picomolar sensitivity
Fastest time to results with highest picomolar sensitivity
Screen for hits and optimize leads based on affinity
Finding true hits faster is the most important step in making your drug discovery workflow efficient. With Dianthus, you’ll find hits quickly and move on to hit validation confidently, whether it’s fragment-based or small molecule single-dose screening.
Spend less time sorting through the strong and weak binders. Dianthus generates easy-to-interpret affinity ranking tables and histograms to help you quickly decide on the right candidates and start lead optimization sooner.
Once validation is complete, it’s time to improve target specificity, selectivity, and potency. Use Dianthus to verify that binding affinities remain strong. This, combined with your ADME, toxicity, and PK/PD results, ensures you’re developing the best drug candidates.
Use proven technology that’s been around for over 10 years
Quantifying molecular interactions — measuring how tight or weak a ligand binds to its target — via Temperature Related Intensity Change (TRIC) isn’t new. It’s done by labeling your target molecule with a fluorescent dye and mixing it with your ligand. Then, a very precise and brief laser-induced temperature change is applied, causing a variation in fluorescence intensity which is amplified if your ligand binds to your target. This change in fluorescence is measured and plotted against your ligand concentration to obtain the dissociation constant or Kd.
Decide which hits are worth moving forward with sooner
Generating results is great, but getting automated, actionable insights from your results is even better. Dianthus DI.Screening Analysis software gives you screening summaries as well as easy-to-interpret ranking tables and histograms. Quickly compare Kds and decide which candidates are worth moving forward with sooner rather than later.
Use high-quality consumables for fast hit screening
Get the high quality and consistency you expect from consumables when finding and validating hits. Dianthus 384-well plates have a proprietary coating that prevents protein from sticking to the wells, and they go through rigorous QC testing to ensure consistency from well to well. So, it’s no wonder the plate’s barcode helps you track your assay and data back to a specific plate. You’ll also want to use a 2nd Generation labeling kit — it labels your proteins or peptides with fluorophores selected for their sensitivity to binding events — so you get the best results.
If you’re looking to get your hit screening assay up and running quickly, use the Buffer Exploration Kit. The buffer plate provides a systematic approach to assay development — it’s pre-loaded with buffer systems that contain various salts, detergents, and additives. Simply mix any of these buffer combinations with your sample to quickly find the right buffer conditions. Finally, reduce the time and cost of assay development.
Do more than screening
Little did you know that Dianthus is capable of doing more than just hit ID and validation. Because it handles a wide range of targets and has flexible throughput, it’s so easy to choose Dianthus as your primary tool for characterization of molecular interactions for a variety of applications.
Characterize binding events to understand biological processes and structure-function relationships
Analyze how multimeric proteins, GPCRs or aptamers interact with their ligands
Support and confirm X-ray crystallography and Cryo-EM findings
Perform competition assays in the presence of inhibitors