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2nd Generation Protein Labeling Kits

Catalog #
Product
Description
MO-L011

MO-L011

Protein Labeling Kit RED-NHS 2nd Generation (Amine Reactive)

The Monolith Protein Labeling Kit RED-NHS 2nd Generation kit increases data quality and enables affinity studies with previously challenging targets by generating higher binding amplitudes and improved signal-to-noise ratios. It contains all required reagents for labeling and dye removal. The dye carries a reactive NHS-ester group that reacts with primary amines (lysine residues) to form a covalent bond. The kit is optimized for proteins with a molecular weight higher than 5 kDa and concentrations between 2 and 20 µM. RED dyes are suited for Monolith instruments with a RED detector (Nano and Pico) and Dianthus instruments.

MO-L014

MO-L014

Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive)

The Monolith Protein Labeling Kit RED-MALEIMIDE 2nd Generation kit increases data quality and enables affinity studies with previously challenging targets by generating higher binding amplitudes and improved signal-to-noise ratios. It contains all required reagents for labeling and dye removal. The dye carries a reactive maleimide group that reacts with cysteine residues to form a covalent bond. The kit is optimized for proteins with a molecular weight higher than 5 kDa and concentrations between 2 and 20 µM. RED dyes are suited for Monolith instruments with a RED detector (Nano and Pico) and Dianthus instruments.

MO-L018

MO-L018

His-Tag Labeling Kit RED-tris-NTA 2nd Generation

The Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit increases data quality and enables affinity studies with previously challenging targets by generating higher binding amplitudes and improved signal-to-noise ratios. The kit is used for site-specific and purification-free labeling of His-tagged (6xHis) proteins and peptides. The kit can be used with purified as well as crude samples (e.g. cell lysate) and contains material sufficient for 500 single-point MST measurements. RED dyes are suited for Monolith instruments with a RED detector (Nano and Pico) and Dianthus instruments.

MO-L019

MO-L019

SNAP-Tag® Labeling Kit RED 2nd Generation

The SNAP-Tag® Labeling Kit RED 2nd Generation contains all required reagents for labeling and dye removal. The dye carries a benzylguanine group that reacts with the SNAP-tag® of your protein of interest to form a covalent bond. The kit is optimized for protein concentrations of 10 µM. Just like all of our 2nd Generation labeling kits, it increases data quality and enables affinity studies with previously challenging targets by generating higher binding amplitudes and improved signal-to-noise ratios. RED dyes are suited for Monolith instruments with a RED detector (Nano and Pico) and Dianthus instruments. Contents require storage at 4 °C and -20 °C.

NT-L111

NT-L111

Large Volume Protein Labeling Kit RED-NHS 2nd Generation (Amine Reactive)

This Large Volume Protein Labeling Kit RED-NHS 2nd Generation kit increases data quality and enables affinity studies with previously challenging targets by generating higher binding amplitudes and improved signal-to-noise ratios. It contains all required reagents for labeling and dye removal. The dye carries a reactive NHS-ester group that reacts with primary amines (lysine residues) to form a covalent bond. The kit is optimized for proteins with a molecular weight higher than 5 kDa and concentrations between 2 and 20 µM. RED dyes are suited for Monolith instruments with a RED detector (Nano and Pico) and Dianthus instruments. Contents require storage at 4 °C and -20 °C.

NT-L114

NT-L114

Large Volume Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive)

This Large Volume Protein Labeling Kit RED-MALEIMIDE 2nd Generation kit increases data quality and enables affinity studies with previously challenging targets by generating higher binding amplitudes and improved signal-to-noise ratios. It contains all required reagents for labeling and dye removal. The dye carries a reactive maleimide group that reacts with cysteine residues to form a covalent bond. The kit is optimized for proteins with a molecular weight higher than 5 kDa and concentrations between 2 and 20 µM. RED dyes are suited for Monolith instruments with a RED detector (Nano and Pico) and Dianthus instruments. Contents require storage at 4 °C and -20 °C.

NT-L118

NT-L118

Large Volume His-Tag Labeling Kit RED-tris-NTA 2nd Generation

This Large Volume His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit increases data quality and enables affinity studies with previously challenging targets by generating higher binding amplitudes and improved signal-to-noise ratios. The kit is used for site-specific and purification-free labeling of His-tagged (6xHis) proteins and peptides. The kit can be used with purified as well as crude samples (e.g. cell lysate) and contains material sufficient for 3840 single-point MST measurements. RED dyes are suited for Monolith instruments with a RED detector (Nano and Pico) and Dianthus instruments. Store at -20 °C.

NT-L020

NT-L020

Biotinylated Target Labeling Kit

The Biotinylated Target Labeling Kit is used for the site-directed labeling of any protein or peptide carrying one or multiple biotin tags. It contains material sufficient for labeling 500 single-point spectral shift measurements. The kit includes a protocol for labeling in 15 min with no removal of excess dye required. The Biotinylated Target Labeling Kit is suited for use with Monolith X and Dianthus with Spectral Shift. Contents require storage at -20 °C.

NT-L120

NT-L120

Large Volume Biotinylated Target Labeling Kit

The Large Volume Biotinylated Target Labeling Kit is used for the site-directed labeling of any protein or peptide carrying one or multiple biotin tags. It contains material sufficient for labeling 4,000 single-point spectral shift measurements. The kit includes a protocol for labeling in 15 min with no removal of excess dye required. The Large Volume Biotinylated Target Labeling Kit is suited for use with Monolith X and Dianthus with Spectral Shift. Contents require storage at -20 °C.

NT-L030

NT-L030

Small Volume Human Fc Labeling Kit

The Small Volume Human Fc Labeling Kit provides a convenient means for the site-specific, purification-free labeling of humanized antibodies or antibody fragments containing the Fc region with fluorescent dye optimized for affinity measurements done with Spectral Shift technology. The kit is used for the site-directed labeling of any protein carrying a Human Fc antibody region. It contains material sufficient for labeling for 500 single-point Spectral Shift measurements. The kit includes a protocol for labeling in <30 min; no removal of excess dye required. The Small Volume Human Fc Labeling Kit is suited for use with Monolith X and Dianthus with Spectral Shift. Contents require storage at -20 °C.

NT-L130

NT-L130

Large Volume Human Fc Labeling Kit

The Large Volume Human Fc Labeling Kit provides a convenient means for the site-specific, purification-free labeling of humanized antibodies or antibody fragments containing the Fc region with fluorescent dye optimized for affinity measurements done with Spectral Shift technology. The kit is used for the site-directed labeling of any protein carrying a Human Fc antibody region. It contains material sufficient for labeling for 4,000 single-point Spectral Shift measurements. The kit includes a protocol for labeling in <30 min; no removal of excess dye required. The Large Volume Human Fc Labeling Kit is suited for use with Monolith X and Dianthus with Spectral Shift. Contents require storage at -20 °C.

Assay Development Kits

Catalog #
Product
Description
NT-B001

NT-B001

Buffer Exploration Kit

Your mission is to find the right buffer conditions for biophysical interaction assays, protein stability, purification, and storage. This kit will help you with your MST and TRIC assay development. Start your buffer exploration with this 96 deep-well plate containing 1 mL of each buffer condition per well.

MO-X001

MO-X001

Monolith Explorer Kit – Mission to Mars

The goal of this mission is to get your assay development going. We’ve packed everything you’ll need to perform one independent labeling reaction for proteins and peptides with RED-NHS and RED-tris-NTA 2nd Generation dyes. Includes dyes, dye removal reagents, and 60 premium capillaries. Store contents at room temperature, 4 °C and -20 °C as required.

Dianthus Plates

Catalog #
Product
Description
DI-P001A

DI-P001A

Dianthus 384-well plate, barcoded – Single plate

Single 384-well barcoded plate for TRIC measurement with Dianthus system. Hard sided, black/black with clear bottom, 18-25 μL working volume.

DI-P001B

DI-P001B

Dianthus 384-well plates, barcoced – Package of 10

Rack of 10 384-well barcoded plates for TRIC measurement with Dianthus system. Hard sided, black/black with clear bottom, 18-25 μL working volume.

DI-P001C

DI-P001C

Dianthus 384-well plates, barcoded – Package of 40

Four racks of 10 384-well barcoded plates for TRIC measurement with Dianthus system. Hard sided, black/black with clear bottom, 18-25 μL working volume.

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