Microscale Thermophoresis in Drug Discovery

Tanja Bartoschik, Melanie Maschberger, Alessandra Feoli, Timon André, Philipp Baaske, Stefan Duhr & Dennis Breitsprecher

Applied Biophysics for Drug Discovery | John Wiley & Sons Ltd. | Donald Huddler & Edward R. Zartler
2017 Chapter: 5 pp: 73-92

Abstract

Biophysical tools are essential for the discovery, development, and characterization of therapeutic proteins and drugs. Over the last decades, the costs of drug discovery and development have seen a considerable increase, owing to an increase in the complexity of target molecules and targeted signaling pathways, as well as higher demands on drug safety and quality. Therefore, novel biophysical techniques are required to reduce costs, time, and assay complexity while providing high information content data in order to boost the efficiency of drug discovery campaigns. Here we describe the use of MicroScale Thermophoresis (MST) for the analysis and quantification of biomolecular interactions and show how the unique properties of MST can be used to rapidly generate binding data with high‐precision and information content while minimizing time and sample consumption. Application examples, MST‐specific benefits, and the integration of MST into fully automated workflows will be discussed.

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Topics: Complex samples, Ions, Physical basics MST, Screening, Small molecules, Monolith – MicroScale Thermophoresis, MST, Proteins, Publications