Are you following your trusted protocol but still getting inconsistent results? The quality of your protein sample could be to blame.
Protein quality is important, especially because more complete information about the quality of your protein — including its presence, purity, concentration, structural integrity, similarity, and functionality — will help you make better decisions on whether to continue using your sample in further experiments. Essentially, the best quality material gives you the highest probability of getting consistent results when following the same protocol.
When working with a protein sample you isolated or were handed, it’s always important to ask: Are there impurities in my sample? Could the addition of salts, the pH or the buffer affecting my protein? Did I use the right amount of material required in the protocol? Is my protein definitely present in the sample prep? Were the samples stored properly? Is the batch of protein I’m using for this experiment similar to the last one I tested?
Tycho, the everyday solution for a quick check of protein quality, provides a detailed snapshot of protein quality early on by examining your protein’s structural integrity, or foldedness.
This will help you decide whether to move forward with your experiments when working with any protein, especially if you are questioning or unsure about the quality of the material you’re working with.
Learn more about quickly identifying the quality of any protein with Tycho. Then, read the protein quality guide to learn more about how and why a quick protein quality check is imperative for any experiment. Bottom line: a quick protein check should be a key part of your daily workflow.