Tackling reproducibility issues in your research by knowing protein quality first

Researchers face many hurdles in science. One of the greatest challenges they face is the reproducibility crisis. “One root cause of irreproducible results is a lack of understanding of the importance of sample quality,” according to Science.


From this report, more than half of the researchers surveyed revealed that they struggle to reproduce not only the results of fellow scientists but even their own experimental data.


Fortunately, implementing technologies into protein purification or characterization workflows can help to quickly analyze sample quality. For example, the Tycho NT.6 system, reveals a lot about the quality of a protein — presence, purity, concentration, functionality, and similarity — in a single experiment. This can all be measured by determining whether your protein is structurally intact or properly folded.

It’s particularly important to compare the structural integrity or unfolding profile of a new sample prep to your reference batch of the right quality to verify that they’re similar. If they differ, you might have a sample of lower quality that contains contaminants, which means you must further purify or optimize your purification workflow. At the end of the day, you’ll achieve more consistent results by working with the highest quality samples.

The earlier you know more complete information about the quality of your protein, the easier it’ll be for you to decide whether or not to move forward with your experiments.

Learn more about quickly identifying the quality of any protein with Tycho. Then, download the Tycho brochure to get a comprehensive view of how any workflow will improve with a quick protein quality check.