How Tycho achieves excellent inter- and intra-assay precision

When it comes to something as important as examining the quality of a protein sample, there’s no room for error.

Intra-assay precision, known as repeatability, describes how similar results are when always obtained by the same operator or the same measuring system in the same lab. It involves trusting the run today and as well as tomorrow. Inter-assay precision, known as intermediate precision, describes how similar results are when obtained within the same laboratory, by different operators, or across measurement systems. This involves trusting or comparing data between different instruments.

Tycho NT.6 provides a detailed snapshot about the quality of your protein — including presence, purity, concentration, functionality and similarity — in a single experiment. Tycho generates thermal unfolding profiles and identifies the inflection temperature that represents unfolding transitions or discrete changes in a protein’s structural integrity. This essentially helps validate the quality of your sample for future experiments.

In a recent test, Tycho achieved excellent repeatability and intermediate precision, proving the instrument to be extremely reliable and an easy-to-validate system for every lab working with proteins. Factors such as repeatability and intermediate precision are particularly important when choosing and selecting an instrument if you share samples or data between labs or sites.

Tycho, the everyday solution for a quick check of protein quality, generates informative data in just three minutes and can help you determine whether your sample material will make or break your next experiment.

Find out how Tycho performs when it comes to repeatability and intermediate precision by reading this technical note.