Use stability profiling to build more effective gene therapy vectors with Prometheus Panta
When you’re developing a gene therapy vector, you need data that gives you confidence in its serotype, that you’ve chosen the most optimal formulation conditions, that your preparation is pure, and that you know how much genetic material it carries. And because vector production is a low-yield and expensive process, you’re faced with getting all of this data without much sample.
Prometheus Panta gives you detailed information about your vectors without wasting material. Its clear-cut, easy-to-interpret stability and characterization results help you truly understand your AAV.
Prometheus Panta gives you the information you need to profile your gene therapy vectors
Save sample for other assays by measuring thermal stability, genetic fill, and purity of your vectors in one experiment.
Get high-quality data about your vector’s stability and behavior that enables you to make the right decisions for its development.
Expedite your decision-making with easy-to-interpret results from intuitive analysis software.
Improve outcomes for your gene therapy vectors with stability and characterization profiling
Whether you’re early in the development process, optimizing your gene therapy vectors, or are concerned with long-term quality assessments, Prometheus Panta ranks your stability parameters and reveals the biophysical attributes of your viral vectors for gene therapy.
Thermal profiling with nanoDSF allows you to confirm batch-to-batch similarity in your AAV production scheme. Also, see how production changes affect the overall thermal stability of your preparations.
Find which formulation conditions provide the best stability to your vectors with nanoDSF and backreflection. Changes to the buffer or stabilizing mutations in viral particle proteins affect how long your products last in cold storage and how efficiently they deliver their genetic payload.
DLS shows you how clean your preparations are, so that you are confident you’re working with high-quality product. Insufficient clean-up processes can lead to unwanted byproducts that lead to lowered efficacy of your gene therapy products.
Both nanoDSF and SLS are tools to monitor the amount of genetic material a vector carries. Creating the perfect therapeutic vector requires you to not only optimize its capsid, but also determine if it has the necessary genetic payload.
SLS determines the molecular weight of your particles to ensure they have assembled correctly. Proper assembly of your AAVs is necessary for packaging your gene therapy products.
Prometheus Panta allows you to compare your preps throughout every step of the workflow, and ensure you’re working with a high-quality, stable product. Storage conditions have an impact on the efficacy of your gene therapy vectors.
Gain unprecedented insight into your gene therapy samples with four integrated technologies
For the first time, monitor the thermal stability and purity of your samples with all these technologies in a single experiment, along an entire thermal ramp
Dynamic Light Scattering (DLS)
Get information about vector size and purity
nano-Differential Scanning Fluorimetry (nanoDSF)
Serotype your vectors and optimize their stability
Backreflection
Monitor the formation of large aggregates
Static Light Scattering (SLS)
Find the molecular weight of your vectors to monitor the amount of genetic material they contain
See what parameters Prometheus Panta
gives you, and how they help your gene therapy vector characterization
nanoDSF
THERMAL UNFOLDING
Tm (for 330 nm, 350 nm, and ratio)
Melting temperature, or point at which 50% is unfolded; higher Tm values represent more thermostable candidates
Tonset (for ratio)
Temperature at which unfolding begins; later onsets indicate greater stability
Ea(*parameter derived from data)
Activation energy of unfolding
Reversibility of unfolding
Point at which protein aggregation prevents re-folding
Dynamic Light Scattering (DLS)
SIZE ANALYSIS
Tsize
Temperature at which average particle size begins to increase; this parameter informs you about vector leakage, as well as overall capsid stability
rH
Hydrodynamic radius shows size of a protein or vector in its solvated state
PDI
Polydispersity index represents distribution of size populations, and can be used to determine how pure a sample prep is
kD
Diffusion interaction identifies impact on colloidal stability; particles that tend to self-associate are more prone to aggregation during storage
Static Light Scattering (SLS)
SIZING INFORMATION
Molecular weight
Average molecular weight of all particles in solution
Tscattering
Temperature at which scattering intensity begins to change, indicating increased size and aggregation
B22
Second virial coefficient used to extrapolate self-association behavior at higher concentrations
Backreflection
AGGREGATION
Tturbidity
Onset temperature of large amorphous aggregates
Become more efficient with simultaneous measurements
Obtaining results that enable efficient but sound decision-making gets more complicated when you have a limited amount of sample, but need to measure multiple parameters.
Exemplary data comes from superior engineering
Specialized optics enable better detection of sample fluorescence, resulting in more precise, accurate data.
And for light scattering applications, Prometheus Panta offers unmatched reproducibility between measurements.
Your samples are loaded into capillaries that are in direct contact with the thermal element, permitting exemplary temperature control throughout the thermal ramp.
Be confident you’re working with the best quality vectors
with precise,
high-resolution data
Prometheus Panta supplies high-quality data to ensure you consistently deliver reliable results.
Design and run your experiments with control software that gives you modular assay set-up, so that you can screen lots of vectors. Select just a single experiment type or combine several assays to build full biophysical profiles of your AAVs.
Expedite your data analysis with software that makes ranking your critical parameters easy, and displays the data with a clean visual interface that’s easy to customize. Export your data in a variety of formats, whether you’re logging it in your electronic lab notebook or presenting results at a conference.
