General Specifications |
Prometheus Panta
|
Prometheus Panta + Robotic Autosampler
|
---|---|---|
Sample volume |
10 µL |
|
Sample handling format |
Individual capillaries or capillary chip |
Capillary chip |
Throughput in one run |
Up to 48 capillaries or 24 in capillary chip |
64 capillary chips (1536 samples, four 384-well plates) before need to reload |
Temperature range |
15 – 110 °C |
|
Heating rate range |
0.1 – 7 °C/min |
|
Precision of 1 °C/min thermal ramp |
± 0.1 °C |
|
Optional high-throughput |
Yes |
|
Dimensions |
35 cm W x 51 cm H x 52 cm D |
110 cm W x 188 cm H x 90 cm D (stand-alone enclosure) |
Weight |
35 kg |
200 kg |
Sample volume
Sample handling format
Throughput in one run
Temperature range
Heating rate range
Precision of 1 °C/min thermal ramp
Optional high-throughput
Dimensions
Weight
10 µL
Individual capillaries or capillary chip
Up to 48 capillaries or 24 in capillary chip
15 – 110 °C
0.1 – 7 °C/min
± 0.1 °C
Yes
35 cm W x 51 cm H x 52 cm D
35 kg
10 µL
Capillary chip
64 capillary chips (1536 samples, four 384-well plates) before need to reload
15 – 110 °C
0.1 – 7 °C/min
± 0.1 °C
110 cm W x 188 cm H x 90 cm D (stand-alone enclosure)
200 kg
Technical Specifications |
Prometheus Panta |
|
---|---|---|
nanoDSF |
Included |
|
Measurement parameters |
ratio: Tonset, Tm, Ea, reversibility of unfolding 330 nm, 350 nm: Tm |
|
Concentration range |
0.005 mg/mL – 250 mg/mL |
|
Inflection point precision @ 75 °C |
± 0.1 °C |
|
Initial ratio repeatability |
0.008 |
|
DLS |
Included |
|
Measurement parameters |
Tscattering, Tsize, rH, PDI, kD |
|
Laser wavelength |
405 nm ± 5 nm |
|
Concentration range (DLS/SLS) |
0.5 mg/mL for a 15 kDa protein, up to 40% w/v |
|
Size resolution |
Down to 0.5 nm – 2 μm |
|
SLS |
Included |
|
Measurement parameters |
Tscattering, average scattering intensity, molecular weight, B22 |
|
Measurement accuracy |
≤ 10% molecular weight |
|
Backreflection |
Included |
|
Measurement parameters |
Tturbidity |
|
Measurement accuracy |
Larger than 12.5 nm radius |
Measurement parameters
Concentration range
Inflection point precision @ 75 °C
Initial ratio repeatability
Measurement parameters
Laser wavelength
Concentration range (DLS/SLS)
Size resolution
Measurement parameters
Measurement accuracy
Measurement parameters
Measurement accuracy
Included
ratio: Tonset, Tm, Ea, reversibility of unfolding 330 nm, 350 nm: Tm
Excitation: 280 nm
0.005 mg/mL – 250 mg/mL
± 0.1 °C
0.008
Included
Tscattering, Tsize, rH, PDI, kD
405 nm ± 5 nm
0.5 mg/mL for a 15 kDa protein, up to 40% w/v
Down to 0.5 nm – 2 μm
Included
Tscattering, average scattering intensity, molecular weight, B22
≤ 10% molecular weight
Included
Tturbidity
Larger than 12.5 nm radius