Just like Dianthus, Monolith NT.Automated can help you with your screening projects — performing both single-dose and affinity screens. Like Monolith, it uses MST to measure binding events in capillaries, but to match the demands of higher throughput, they come in chips holding 24 capillaries each.
Higher throughput, detection versatility and automation option for your screening projects
When you need to increase your manual throughput
Load up to 4 capillary chips per run for up to 8 Kds in only 30 minutes
When you want flexibility for your labeling strategy
Choose up to 4 fluorescent channels, including label-free and pico options
When you need to measure very strong affinities
A pico option gives you extended sensitivity to pM levels
When you want to have a path to full automation and walk-away operation, now or later
Combine with the NT.Automated Screening unit to automate liquid handling as well as capillary chips filling and loading, for hours of unattended operation
Monolith NT.Automated has no fluidics — that means no maintenance
Choose from up to 4 fluorescent channels plus label-free for the highest detection versatility. When your projects demand automation, get the Screening Unit for automated liquid handling and capillary chip loading with hours of unattended screening.
A great choice for handling both single-dose and affinity screens with the most fluorescent options to choose from. Provides a path to automation should your projects require even more hands-off throughput.
Plus NT.Automated Screening Unit
Add this if you want hours of unattended operation including automated liquid handling and capillary chip filing and loading.
Clearly identify false positives at early stages of your discovery process
Count on Monolith NT.Automated to run your single-dose and affinity screen campaigns. Because it uses MST to measure interactions, the single-dose screen identifies not only hits, but also gives you information about compound‐induced aggregation — super important to keep false positives from entering later stages of the drug discovery process. Additionally, you don’t have to modify the automated workflow of the subsequent affinity screen, because all the steps can be done using the same automation setup used in the single-dose screen.
A single-point screen of small molecule inhibitors with labeled p38 alpha kinase identified five hits A through F (upper panel, dotted lines represent hit thresholds). Next, an affinity screen measured the binding affinities for the 5 hits (lower panel). Four of them had Kds in the nanomolar range, and one was determined to be a non-binder (F).
From Bartoschik, T., et al. Applied Biophysics for Drug Discovery. Chapter 5: Microscale Thermophoresis in Drug Discovery (2017)
Software that complements your screening projects
MO.Screening Control makes screening assays setup accessible to everyone in the lab. For those large data sets from screening experiments, MO.Screening Analysis software just might be the right thing to add to your tool box.
Get similar step-by-step guidance that made MO.Control popular. MO.Screening Control, designed specifically for screening experiments, helps with planning sample layout on either 96- or 384-well plates, defining measurement settings and performing measurements.
Let MO.Screening Analysis automatically evaluate and identify the hits among thousands of ligands from single dose screens. Identify strong and weak ligands by reporting Kds in affinity-based screenings.
Get great results with tailor-made consumables
Monolith NT.Automated uses capillary chips that adapt perfectly to robotic handling and manual loading alike. The capillaries are manufactured in a state-of-the-art facility and rigorously tested before they are put into the chips. Use Monolith Protein Labeling Kits to get the highest quality data and ultimately, the best outcome.