With Monolith X, you’ll get Spectral Shift and MST — two biophysical modalities to help cover all the types of interactions you encounter. You’ll enjoy high-quality results without spending your time on assay development and finally perform experiments without worrying about sample aggregation or impurities.
For one reason or another, SPR has a difficult time analyzing challenging binding events no matter how many times you’ve tried to develop an assay. When you find yourself in these challenging situations, turn to Monolith to help.
It’s common practice to validate your results with more than one technique because you want to be confident that the results are real. Monolith, with its immobilization-free measurement, is the perfect orthogonal tool for SPR users. It removes the immobilization bias and helps to confirm your results, identify false positives, or find binding partners that your primary assay missed.
Rely on Monolith’s versatile capabilities to execute immobilization-free experiments quickly and efficiently. Tackle projects that involve almost any molecule, buffer composition, or binding strength, all while consuming only a small amount of sample.
Work with almost any molecule including IDPs, membrane proteins, large protein complexes, PROTACS, small molecules or ions.
Evaluate results independently of size and mass differences in binding partners.
Analyze the binding of purified and crude samples without worrying about interference from buffer additives like detergents.
Measure a broad range of binding affinities, from pM to mM, allowing you to detect strong and weak binders.
Scientists use Monolith to study binding stoichiometry* and thermodynamic parameters*, assess relative affinities with competition assays, and characterize binding cooperativity*.
*Requires offline data handling, not supported by Monolith software
Because Monolith doesn’t use fluidics, doesn’t require warm-ups, calibrations, washes, cleaning or flushing in between runs, you are able to run assays whenever you want.
You’ll be ready to run experiments independently after only one day of training. Monolith’s software takes you through step-by-step instructions on how to prepare and run your assay – making it a straightforward experience for anyone on your team.
with Spectral Shift and MST technologies
Monolith X is equipped with Spectral Shift and MST technologies and provides you high quality data with little to no assay development.
with MST technology
Monolith comes with MST and enables you to measure high affinity interactions in the pM range, work label-free, or use GFP-tagged proteins.
Monolith comes with two biophysical modalities — Spectral Shift and MST — that help you measure the strength of your molecular interactions. Experiments start by labeling one molecule with a fluorophore*, then mixing a fixed amount of it with a dilution series of the binding partner. Samples are then loaded into capillaries and are placed into the instrument for the measurements to start.
*Alternatively, the intrinsic fluorescence of tryptophan can be used for MST.
Most software begins with loading a sample and starting the measurement. Monolith’s software is built differently — not only does it provide step-by-step experimental planning and assay setup guidelines, but it also provides immediate feedback on assay optimization based on the results. In addition, you have the option to merge data sets and group them for comparison purposes, to get results that are reported with presentation-worthy data and publication-ready figures.
Monolith capillaries are manufactured in a state-of-the-art facility and rigorously tested. For the highest success, pair the capillaries with a Monolith Protein Labeling Kit to get the highest quality data and ultimately the best outcome.
With the online and interactive Protein Labeling Assistant, you’ll get the best labeling recommendations for proteins and instrument optics.
Make buffer screen less time-consuming with the Buffer Exploration Kit, a 96-well plate loaded with ready-to-use buffers.