Bromodomains (BRDs) have an important role in the targeting of chromatin-modifying enzymes to specific sites that contain acetyl lysines. As the “readers” of lysine acetylation they transduce the signal encoded by modified lysines into a phenotype. Proteins that contain BRDs have been implicated in the development of a large variety of diseases like cancer and inflammation. DMSO has been shown to disrupt the Bromodomain-ligand interactions. Thus, DMSO in the assay buffer should be avoided or its concentration kept as low as possible (below 0.05% v/v).