The Buffer Screen experiment template in DI.Control relies on the use of the NanoTemper Technologies Buffer Exploration Kit (Cat# NT-B001). The goal of assay development for interaction-based screening methods is finding the conditions that ensure the best signal- to-noise ratio and assay stability over time. The search for these optimal assay conditions is a long and costly process due to the large number of variables that have to be tested. Access to a systematic approach that examines a large number of variables at once can reduce the complexity of assay development for interaction-based screening methods — which translates into a faster and less costly process. The NanoTemper Technologies Buffer Exploration Kit (Cat# NT-B001) provides a systematic, plate-based approach for assay development in interaction- based screening. With this method, 96 different buffer conditions can be tested at once. An application example, the kit composition as well as manuals for optimal use can be found in the NanoTemper Explorer Community.