Reference Sample

In the context of a Single-Dose Screening, a reference sample corresponds to the Target in the unbound (see Baseline) state. Oftentimes the reference sample is the target alone in the final Buffer (including the corresponding final DMSO concentration). We strongly recommend running reference samples in at least 4-8 wells (evenly distributed over the Plates to account for time-effects) on every plate in single dose screenings.

In Affinity Screening, reference samples are less common because the titration Baseline usually accounts for that.