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Dianthus NanoPedia Specificity of an interaction

Specificity of an interaction

In rare cases, increasing concentrations of a ligand can cause changes in TRIC signals that are not specific (not binding-induced). To discriminate between specific and unspecific effects on the Response Amplitude, increase the ligand concentration even further. If saturation can be reached, the effect is specific. In case your sample exhibits a Ligand-Induced Fluorescence Change perform the appropriate Specificity Test to find out whether the observed fluorescence effect is in fact due to binding.

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