Start getting consistent results
Finding out the quality of your protein sample upfront will help you figure out if it’s functional, similar to a previous batch you made or if it’s been properly stored. With very little effort and a tiny amount of sample, quickly test your protein’s quality early and throughout your workflow to make sure you’re moving forward with a viable sample.
Quickly verify protein quality
Ever wonder if your protein is the same as it was a day or a year ago? Is it even present? Confirm sample quality at any point in your workflow so you know you still have the right protein.
Get answers in minutes
Generate informative data in 3 minutes fast — it makes deciding what to do next that much easier.
Easily analyze any protein sample type
Forget dialyzing or doing sample dilutions. Determine the quality of any protein in any type of buffer over a wide range of concentrations.
Conserve precious sample
Purifying protein for the umpteenth time because you’ve run out of sample is painful. Not only can checking your protein’s quality help you run less experimental parameters later, but it only requires microliters of sample for analysis.
Small benchtop footprint
Save scarce bench space for handwork while getting valuable info from a powerful system with a tiny footprint.
Confirm protein quality with Tycho NT.6
Integrate quality checks into your workflow whenever it makes sense. Not sure why you’re getting inconsistent results even though you followed the same protocol? Check your samples with Tycho. Want to quickly find out if your protein is present? Check your samples with Tycho. You get the picture 🙂 Find out if Tycho is right for you >
Hit the bench running
Using Tycho is super easy — just follow the onscreen prompts to start an experiment. Assign names to your samples while your experiment is running to keep your records in order. When it’s done, simply export both the raw data, automatically analyzed results, and summary figures in a convenient ready-to-share format.
Small amounts of sample is all you need
You can do so much (or so little) using Tycho and minute amounts of sample. Accurate results are obtained using tiny, precisely constructed glass capillaries so analysis can be done on virtually any sample type. No pipetting is required since sample is taken up by capillary force directly into the capillary, so you’re not wasting your precious sample.
Tycho NT.6 was adopted by all our lab members in no time, providing crucial quality information not detectable before. It will become a standard tool for everyone working on protein biochemistry.
Dr. Gregor Witte
Principal Investigator, K.P. Hopfner lab, Ludwig-Maximilians-University of Munich
How does Tycho identify sample quality?
Tycho verifies protein quality by looking at the structural integrity (or foldedness) of a protein. As a thermal ramp is applied, the changes in the intrinsic fluorescence, detected at both 350 nm and 330 nm, from tryptophan and tyrosine residues in the protein are measured. These changes in fluorescence signal indicate transitions in the folding state of a protein. The temperature at which a transition occurs is called the inflection temperature (Ti). The fluorescence is recorded during the thermal run, plotted as ratio and used to calculate Ti. It’s initial value along with the change in signal over the run (Δ ratio) is also recorded.
Check for inconsistencies
It’s critical to know if you have inconsistencies from batch to batch or if your protein samples were stored correctly if you’re concerned with reproducible results. Compare multiple preparations or various test conditions to reference data so you can be confident that your samples are good before committing time and effort in lots of work downstream.
More efficient assay optimization
Assay development gets tedious real quick — you’re testing all those different conditions, often without a proper readout unless your experiment fails. It can take a lot of time to determine the most optimal conditions. But, Tycho can help you screen various buffers in minutes, easily comparing different conditions against a reference standard. Make clear decisions on which buffers maintain the quality of your target protein before you even start your assay.
Know protein quality early on
During monoclonal antibody therapeutic development, knowing structural integrity as early as possible is one of the strongest indicators of functionality and potential drug efficacy.
A monoclonal antibody (Herceptin) was treated with two oxidation conditions and run on Tycho to test the effects of the treatment on sample quality. A longer oxidation treatment caused more unfolding of the mAb as indicated by the increased initial ratio reading. In addition, increased oxidation treatment causes a left shift in the detected first inflection temperature suggesting possible structural changes to particular domains in the protein (left). MicroScale Thermophoresis (MST) was performed to confirm the effect oxidation treatment can have on Herceptin binding affinity (right).
Find out if Tycho is right for you
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